Table 1.

The cutoff criteria for evaluation of true positive and false-positive rates.

		Cutoff (number of sgRNAs with 4-fold reduction)
	Total	>1	>2	>3	>4
Essential genes	216	138	67	25	5
Non-essential genes	771	105	15	2	0
False-negative rate:		36.1%	69.0%	88.4%	97.7%
False-positive rate:		13.6%	1.9%	0.3%	0.0%