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. 2020 Sep 22;123(12):1737–1748. doi: 10.1038/s41416-020-01088-w

Fig. 6. CB11 suppresses the EMT phenotype in radiation-exposed radio-resistant NSCLC cells and induces apoptotic cell death.

Fig. 6

a A clonogenic cell survival assay was conducted with CB11 (30 μΜ, 24 h) treatment after exposure of various radiation doses (0, 2, 4, or 6 Gy). The survival fraction was calculated using the surviving fraction formula in A549, H460, A549R, and H460R cells; *p < 0.05. b, c A549, H460, A549R, and H460R cells were treated with CB11 (30 μΜ, 24 h) after radiation exposure with 2 Gy. Cell viability was determined for these cells, and Western blot analyses for E-cadherin, N-cadherin, vimentin, slug, and snail were conducted. d–f PPARɣ shRNA stable H460R cell lines were established after H460R cells were transfected with PPARɣ shRNA. These cells were exposed to CB11 (30 μΜ), 2 Gy, and CB11 + 2 Gy, and cell viability and LDH assays were performed along with Western blot analyses examining the levels of p-ATM, p-p53, GADD45α and cleaved caspase-3; *p < 0.05. β-actin was used as a protein loading control.