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. 2020 Oct 6;184(4):2154–2167. doi: 10.1104/pp.20.00474

Figure 2.

Figure 2.

CLASP protein levels are diminished in dark-grown root meristems. A and B, Confocal z-projection of root division zone epidermal cells expressing CLASPpro:GFP-CLASP grown in the light (A) and dark (B) for 6 d. Insets show magnified views of GFP-CLASP on cell edges. Scale bars = 30 μm for large images and 10 μm for insets. C, Quantification of fluorescence intensity in the root division zone of CLASPpro:GFP-CLASP/clasp-1-expressing plants transferred from light to dark conditions and vice versa. Asterisks indicate significance assessed by Student’s t test with equal variance, performed to compare days 2 and 4 samples to those on day 0 (***P < 0.001). NS, Not significantly different. Error bars indicate 95% confidence intervals. n > 15 roots for each treatment. D, Confocal z-projection of CLASPpro:GFP-CLASP/clasp-1 plants germinated and grown in the dark for 4 d (day 0), then transferred to 24-h light conditions. Images show CLASPpro:GFP-CLASP 2 and 4 d following transfer to light. E, CLASPpro:GFP-CLASP/clasp-1 plants germinated and grown in the light for 4 d (day 0) then transferred to 24-h dark conditions. Images show CLASPpro:GFP-CLASP 2 and 4 d following transfer to the dark. Scale bars = 30 μm.