Figure 5.

The enhanced seed dormancy and endogenous ABA concentration of ref6-1 depend on the reduced expression of CYP707A1 and CYP707A3. A, The seed germination rates of Col and various mutants. FH seeds with or without ST were used in the experiments. Values (mean ± sd) are from three biological replicates, each consisting of three technical replicates. At least 50 dry mature seeds were used in each technical replication. B, Representative pictures showing germinating seeds at 2.5 d after sowing. FH seeds with ST were sown on one-half strength MS. Scale bar = 0.6 mm. C, The endogenous ABA concentration in FH seeds of Col and various mutants. Values (mean ± sd) are from three biological replicates, each consisting of ∼100 mg of FH seeds. Lowercase letters indicate statistical differences determined using one-way ANOVA with Duncan’s multiple range test (for FH-NST-4D, the F value = 373,910 and degrees of freedom = 13).