Reproductive organs of A. trichopoda and PG isolation procedure. A, Mature female flower consisting of five to six carpels (C) with a wet stigma (S; here the five stigmata are labeled with an asterisk), five to eight tepals (T), and two to three staminoids (St; here one is visible). B, Mature male flower showing 10 to 25 anthers (here 17 are labeled with an asterisk). C and D, Dissected anther (C) and mature PG showing the porus (D). E, Schematic diagram describing the process of PG isolation in five steps as indicated. See text for details. Scale bars = 1 mm (A–C) and 10 μm (D).