Table 1. Overview of RNA-seq data obtained from nine A. trichopoda tissues.
Mapped reads, number of expressed genes (TPM ≥ 1), and preferentially male gametophyte-expressed genes in PG and PT at the PT-Bi and PT-Tri stage. Male flowers (MALE) that contain male gametophytes were excluded from the analysis to identify preferentially expressed genes. Percentage refers to total number of protein-coding genes (27,313 genes; Amborella Genome Project, 2013).
| Tissue Type | Sample | Mapped Reads | No. of Expressed Genes | No. of Preferentially Expressed Genesa |
|---|---|---|---|---|
| Millions | % | % | ||
| Male Gametophytic Tissues | PG | 46.60 | 10,134 (37.10) | 470 (1.72) |
| PT-Bi | 63.88 | 11,384 (41.67) | 652 (2.38) | |
| PT-Tri | 58.01 | 11,002 (40.28) | 611 (2.23) | |
| Flower Tissues | MALE | 80.25 | 15,611 (57.15) | – |
| FEMALE | 81.96 | 16,802 (61.51) | – | |
| OVARY | 8.91 | 6,144 (22.49) | – | |
| TEPAL | 50.17 | 14,396 (52.70) | – | |
| Vegetative Tissues | LEAF | 22.55 | 15,003 (54.93) | – |
| ROOT | 18.60 | 16,174 (59.22) | – |
a
Genes with a TPM ≥ 1 in a given tissue and TPM ≤ 3 in sporophytic tissues (ovary, leaf, tepal and root; see Supplemental Table S4).