Figure 5.
Expression of pEXO70A2:: GFP:EXO70A2 complements defects of the exo70a2 mutant pollen. A, Representative micrographs of in vitro germinated pollen from the wild type and the exo70a2/pEXO70A2::GFP:EXO70A2 line 20 h after imbibition. Bright-field images are shown at left and green fluorescence images at right. B, Pollen germination efficiency 20 h after imbibition in vitro. Error bars represent the mean ± sd (n > 145). Lowercase letters denote statistically significant different groups evaluated by ANOVA (P < 0.01). C, Distribution of pollen tube lengths 20 h after imbibition (n > 60). Ten samples originating from five different plants were evaluated for each genotype, with two biological replicates.