Figure 3.

Constitutive expression of CjFNS I/F2H, CjFNS I1, and SmFNS I/F3H in Arabidopsis. A, Transgene transcript presence as determined by RT-PCR. B, Representative HPLC profiles of flavonoids produced by nontransgenic and transgenic Arabidopsis provided with naringenin as substrate. Api, Apigenin; Kae, kaempferol; Nar, naringenin. C and D, Mass spectrometry profiles of apigenin (C) and kaempferol (D) produced in Arabidopsis. E and F, Kaempferol (E) and apigenin (F) produced by nontransgenic Arabidopsis and three transgenic lines grown in the presence of naringenin. DW, Dry weight. Data are means ± sd of three biological replicates. Statistical analysis was performed with Student’s t test. Means differ from the wild type (WT) at P < 0.05 (*) and P < 0.01 (**).