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. 2020 Oct 6;184(4):1731–1743. doi: 10.1104/pp.20.01185

Figure 4.

Figure 4.

In vitro enzyme activity of mutant and wild-type GbF3H, PsF3H, PrF3H, AtF3H, MtF3H, AtrF3H, PcF3H, and AcF3H, based on naringenin as substrate. A and C, HPLC profiles of enzyme activity of mutant and wild-type GbF3H, PsF3H, and PrF3H in vitro (A) and their peak areas (C). B and D, HPLC profiles of enzyme activity of mutant and wild-type AtF3H, MtF3H, AtrF3H, PcF3H, and AcF3H in vitro (B) and their peak areas (D). P1, Dihydrokaempferol; P2, apigenin; S1, naringenin. Values shown are means ± sd (n = 3). Statistical analysis was performed with Student’s t test. Means differ from the wild-type enzyme at P < 0.05 (*) and P < 0.01 (**).