Table 2. Kinetic analysis of recombinant CjFNS I1 and CjFNS I/F2H using dihydrokaempferol as substrate.
Enzyme | Km | kcat | kcat/Km |
---|---|---|---|
μm | s−1 | m−1 s−1 | |
CjFNS I1 | 13 ± 2.3 | 0.30 ± 0.0091 | 2.3 × 104 |
CjFNS I/F2H | 38 ± 7.9 | 0.042 ± 0.0025 | 0.11 × 104 |
Enzyme | Km | kcat | kcat/Km |
---|---|---|---|
μm | s−1 | m−1 s−1 | |
CjFNS I1 | 13 ± 2.3 | 0.30 ± 0.0091 | 2.3 × 104 |
CjFNS I/F2H | 38 ± 7.9 | 0.042 ± 0.0025 | 0.11 × 104 |