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. 2020 Oct 5;184(4):1979–1997. doi: 10.1104/pp.20.00219

Figure 7.

Figure 7.

Expression levels of ZmRNRL1 and ZmRNRL2. A and B, Comparisons of ZmRNRL1 and ZmRNRL2 expression levels in various tissues of wild-type (A) and tvt1-R plants (B) in the B73 genetic background using sq-PCR. C and D, RT-qPCR analysis of ZmRNRL1 (C) and ZmRNRL2 (D) expression in various tissues of wild-type and tvt1-R plants in the B73 genetic background. Values are means ± sd (n = 3 biological replicates, each with three technical replicates). Two-tailed Student’s t test was used to determine significant differences (*P < 0.05, **P < 0.01, and ***P < 0.001). N, No significant difference. E, Ears; GD, genomic DNA; M, DNA markers; Pt, permissive temperatures; R, roots; Rt, restrictive temperatures; S, ninth leaf-wrapped stems; ST, shoot tips; T, tassels; Ta, tasseling stage at permissive temperatures; TL, topmost visible leaves; V4, V4 stage; V9, V9 stage at permissive temperatures. ZmActin1 was used as the internal control.