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. 2020 Oct 5;184(4):1979–1997. doi: 10.1104/pp.20.00219

Figure 9.

Figure 9.

The rnrl1 mutations probably affect DNA replication at restrictive temperatures. A, Hierarchical clustering of nine downregulated ribosomal protein genes in tvt1-R-B73/tvt1-R-B73 plants compared to Tvt1-B73/Tvt1-B73 plants. B, Hierarchical clustering of six downregulated histone genes in tvt1-m1/tvt1-R plants compared to Tvt1/tvt1-R plants. In A and B, FPKM values of the three biological replicates are shown. C, RT-qPCR validation of eight downregulated ribosomal protein genes in tvt1-R-B73/tvt1-R-B73 plants compared to Tvt1-B73/Tvt1-B73 plants. D, RT-qPCR validation of five histone genes in tvt1-m1/tvt1-R plants compared to Tvt1/tvt1-R plants. In C and D, ZmActin1 was used as the internal control. Values are means ± sd (n = 3 biological replicates, each with three technical replicates). Two-tailed Student’s t test was used to determine significant differences. P values are indicated in the figure. E, Results of flow cytometric analysis of the second leaf tips at V2 in plants of the four different genotypes. The 2C cells, 4C cells, and all cycle events for each genotype are calculated from the sum of three biological replicates. All cycle events of Tvt1-B73/Tvt1-B73, tvt1-R-B73/tvt1-R-B73, Tvt1/tvt1-R, and tvt1-m1/tvt1-R plants are 16,734, 16,237, 17,700, and 17,776, respectively. Statistical analysis of the percentage data between two samples was performed by χ2 test.