Figure 1.

The extra1 mutant shows excessive transpiration due to increased cuticle permeability. A, Thermal imaging of wild type (WT) of Col-0, extra1, and the extra1 transgenic lines expressing a normal, functioning ACC1 gene. B, Cuticle permeability analysis. Aerial parts of wild-type, extra1, and the extra1 transgenic lines expressing ACC1 were stained with 0.1% (w/v) toluidine blue for 5 min. Scale bars = 1 cm. C, TEM images of the cuticle layer of rosette leaves of wild type and extra1 mutant. Scale bars = 100 nm. D, Transpiration rate of the three accessions; slac1-2 (the mutant with stomata always open), and lacs2-3 (the mutant with a thin cuticle). Data presented are means ± se (n ≥ 7).