UV-B irradiation causes cell death in the Arabidopsis SAM. A to D, Top views of the Arabidopsis inflorescence meristem expressing pCLV3::mGFP-ER (green) and 35S::LTI-YFP (red) imaged at 0 h (A), 2 h (B), 8 h (C) and 24 h (D) after 2 min of UV-B exposure, drawn from the confocal Z-section data. E to H, Reconstructed side views of the inflorescence meristem showing the layering organization of the SAM. I to L, Reconstructed side views of the floral meristem. Arrows in B and J indicate cell death in the epidermal cell layer in a stage 4 floral primordium visualized by PI staining (blue). PI marks the outline of cells in live tissue but enters into the dead cells when the membrane integrity is compromised. Arrowheads in F and G indicate cell death in the deeper cell layer of the SAM (blue). Scale bars = 40 μm.