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. 2020 Dec 1;23(2):107. doi: 10.3892/mmr.2020.11746

Figure 2.

Figure 2.

IMD suppresses TG-induced ER stress in cardiomyocytes. Neonatal rat cardiomyocytes were treated with 3 µM TG and the indicated dose of IMD for 24 h. The expression levels of ER stress markers (GRP78, CHOP and caspase-12) were detected by western blotting. (A) Representative western blots for GRP78, CHOP and caspase-12. Semi-quantification of the protein levels of (B) GRP78, (C) CHOP and (D) caspase-12. Data on dose response were analyzed with a one-way ANOVA followed by the Tukey test. Data are presented as the mean ± SD. n=3. **P<0.01; #P<0.05, ##P<0.01. IMD, intermedin; ER, endoplasmic reticulum; TG, thapsigargin; GRP78, 78 kDa glucose-regulated protein; CHOP, C/EBP-homologous protein.