Figure 4.

IMD improves SERCA activity by regulating PLB phosphorylation. Cardiomyocytes were treated with 3 µM TG in the presence or absence of 100 nM IMD for 24 h. p-PLB and t-PLB protein expression levels were assayed by western blot analysis. Representative western blots and semi-quantification of p-PLB and t-PLB. Data were analyzed with a student's t-test for two group comparison. Data are presented as the mean ± SD. n=3. **P<0.01. IMD, intermedin; SERCA, sarco/endoplasmic reticulum calcium ATPase; PLB, phospholamban; p-, phosphorylated; t-, total; TG, thapsigargin.