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. 2020 Dec 1;23(2):107. doi: 10.3892/mmr.2020.11746

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — IMD reduces endoplasmic reticulum stress-mediated apoptosis in cardiomyocytes. Neonatal rat cardiomyocytes were treated with TG in the presence or absence of IMD or H-89 for 24 h. Apoptotic rates were assessed using flow cytometry analysis. (A) Representative flow cytometric dot plots for each treatment group. Horizontal and vertical axis represent Annexin V and PI staining. Lower left quadrant, living cells; upper left quadrant, necrotic cells; and right quadrants, apoptotic cells. (B) Quantitative analysis of apoptotic cells. Data were analyzed with a student's t-test for two group comparison. Data are presented as the mean ± SD. n=3. *P<0.05 and **P<0.01. IMD, intermedin; PI, propidium iodide; TG, thapsigargin.