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. 2020 Nov 25;23(2):92. doi: 10.3892/mmr.2020.11731

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Copyright: © Sayama et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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Figure 2. — Flow cytometric detection of TROP2 using TrMab-6. (A) CHO-K1, (B) CHO/TROP2-PA, (C) MCF7, (D) BINDS-29 (MCF7/TROP2-KO), (E) BT-474, (F) Lec1/TROP2, (G) Lec2/TROP2 and (H) Lec8/TROP2 cells were incubated with TrMab-6 (1 µg/ml; red line) or 0.1% BSA in PBS (grey) for 30 min, followed by Alexa Fluor 488-conjugated secondary antibodies. Fluorescence data were collected using a cell analyzer. TROP2, trophoblast cell-surface antigen 2.