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Detection of TROP2 with TrMab-6 using western blot analysis. The cell lysates (10 µg) of CHO-K1, CHO/TROP2-PA, MCF7, BINDS-29 (MCF7/TROP2-KO), BT-474, Lec1/TROP2, Lec2/TROP2 and Lec8/TROP2 were subjected to SDS-PAGE and transferred onto the PVDF membranes. The membranes were incubated with l µg/ml of (A) TrMab-6, an (B) anti-PA mAb (NZ-1) and an (C) anti-β-actin mAb (AC-15), followed by secondary antibodies. TROP2, trophoblast cell-surface antigen 2.
