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miR-576-5p regulated EMT by targeting Wnt5a. SW480 cells were transfected with miR-576-5p mimic to overexpress miR-576-5p, with or without Wnt5a overexpression or XAV-939 treatment. Protein expression of E-cadherin, vimentin, N-cadherin and Snail in different groups were detected using western blotting. *P<0.05, **P<0.01 and ***P<0.001. miR, microRNA; NC, negative control.
