Figure 4.

let7f-5p targets MAPK6. (A) TargetScan software was used to predict the complementary binding sites between let7f-5p and MAPK6. (B) Reverse transcription-quantitative PCR was performed to assess the transfection efficiency of let7f-5p mimic in A549 and WI-38 cells. Dual-luciferase reporter assays were performed to validate the interaction between let7f-5p and MAPK6 in (C) A549 and (D) WI-38 cells. **P<0.01 and ***P<0.001 vs. NC mimic. NC, negative control; UTR, untranslated region; WT, wild-type; MUT, mutant.