TABLE 2.
The Ct values in the real‐time PCR assay for the ovine and caprine detection in the fresh meats
| Samples | Ct value a | ||
|---|---|---|---|
| Sheep‐FAM | Goat‐HEX | Control‐ROX | |
| Sheep 1 | 20.45 ± 1.53 | 0 | 18.06 ± 0.09 |
| Sheep 2 | 20.91 ± 1.12 | 0 | 18.53 ± 0.25 |
| Sheep 3 | 20.38 ± 0.03 | 0 | 20.87 ± 0.28 |
| Sheep 4 | 20.97 ± 0.34 | 0 | 20.89 ± 0.29 |
| Sheep 5 | 24.06 ± 2.76 | 0 | 24.05 ± 0.73 |
| Sheep 6 | 27.08 ± 2.69 | 0 | 24.51 ± 0.45 |
| Sheep 7 | 21.05 ± 0.83 | 0 | 18.43 ± 0.14 |
| Sheep 8 | 18.28 ± 0.75 | 0 | 18.44 ± 0.91 |
| Goat 1 | 0 | 17.2 ± 0.16 | 17.77 ± 0.72 |
| Goat 2 | 0 | 17.95 ± 0.46 | 17.95 ± 1.43 |
| Goat 3 | 0 | 21.09 ± 0.78 | 19.28 ± 0.87 |
| Goat 4 | 0 | 21.23 ± 1.04 | 19.52 ± 0.7 |
| Goat 5 | 0 | 24.69 ± 0.12 | 25.46 ± 0.35 |
| Goat 6 | 0 | 26.34 ± 1.42 | 26.6 ± 1.08 |
| Goat 7 | 0 | 22.85 ± 0.6 | 23.27 ± 0.19 |
| Goat 8 | 0 | 22.91 ± 1.13 | 23.48 ± 1.14 |
| Beef | 0 | 0 | 18.52 ± 0.12 |
| Horse | 0 | 0 | 21.48 ± 0.41 |
| Pork | 0 | 0 | 19.02 ± 0.49 |
| Chicken | 0 | 0 | N/A |
| Duck | 0 | 0 | N/A |
| Goose | 0 | 0 | N/A |
| Dog | 0 | 0 | N/A |
| Rabbit | 0 | 0 | N/A |
| Cat | 0 | 0 | N/A |
| Fish | 0 | 0 | N/A |
Abbreviation: N/A, not applicable.
a
Data (average ± SD) represent three replicates.