TABLE 4.

The Ct values in the real‐time PCR assay for the sensitivity of identification of meat and dairy products from sheep and goat

Samples	Input DNA amount (ng)	Ct value a
		Sheep‐FAM	Goat‐HEX
Fresh ovine meat	100	14.24 ± 0.21	N/A
	10	17.13 ± 0.35	N/A
	1	21.82 ± 0.96	N/A
	0.1	25.99 ± 0.58	N/A
	0.01	30.40 ± 0.49	N/A
	0.001	35.34 ± 0.90	N/A
	0.0005	0	N/A
	0.00025	0	N/A
	0.0001	0	N/A
	0.00001	0	N/A
Processed ovine meat	100	19.24 ± 0.49	N/A
	10	23.15 ± 0.68	N/A
	1	26.80 ± 1.11	N/A
	0.1	31.32 ± 0.65	N/A
	0.01	36.21 ± 1.10	N/A
	0.005	0	N/A
	0.0025	0	N/A
	0.001	0	N/A
	0.0001	0	N/A
	0.00001	0	N/A
Fresh caprine meat	100	N/A	14.69 ± 0.32
	10	N/A	17.61 ± 0.41
	1	N/A	21.3 ± 2.15
	0.1	N/A	26.03 ± 0.61
	0.01	N/A	30.58 ± 0.55
	0.001	N/A	34.62 ± 1.07
	0.0005	N/A	34.96 ± 1.86
	0.00025	N/A	37.01 ± 1.3
	0.0001	N/A	0
	0.00001	N/A	0
Caprine milk	100	N/A	15.03 ± 0.31
	10	N/A	19.08 ± 0.28
	1	N/A	23.23 ± 0.49
	0.1	N/A	27.36 ± 0.59
	0.01	N/A	32.77 ± 1.13
	0.005	N/A	34.55 ± 1.06
	0.0025	N/A	0
	0.001	N/A	0
	0.0001	N/A	0
	0.00001	N/A	0
Caprine cheese	100	N/A	20.95 ± 0.55
	10	N/A	24.86 ± 0.50
	1	N/A	28.88 ± 0.41
	0.1	N/A	33.79 ± 1.33
	0.01	N/A	37.65 ± 1.06
	0.005	N/A	0
	0.0025	N/A	0
	0.001	N/A	0
	0.0001	N/A	0
	0.00001	N/A	0

Abbreviation: N/A, not applicable.

^aData (average ± SD) represent 20 replicates.