TABLE 5.
The Ct values in the triplex real‐time PCR for the meat and milk mixtures from sheep and goat
| Samples | Volume (%) | Ct value a | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Ovine meat | Porcine meat | Caprine meat | Chicken meat | Caprine milk | Bovine milk | Sheep‐FAM | Goat‐HEX | Control‐ROX | |
| Mix 1 | 99.9 | 0.1 | N/A | N/A | N/A | N/A | 15.49 ± 0.42 | 0 | 15.58 ± 0.19 |
| Mix 2 | 99 | 1 | N/A | N/A | N/A | N/A | 15.17 ± 0.29 | 0 | 15.25 ± 0.14 |
| Mix 3 | 90 | 10 | N/A | N/A | N/A | N/A | 16.98 ± 0.36 | 0 | 16.83 ± 0.14 |
| Mix 4 | 70 | 30 | N/A | N/A | N/A | N/A | 16.76 ± 0.42 | 0 | 16.35 ± 0.13 |
| Mix 5 | 30 | 70 | N/A | N/A | N/A | N/A | 17.42 ± 0.23 | 0 | 15.79 ± 0.1 |
| Mix 6 | 10 | 90 | N/A | N/A | N/A | N/A | 19.9 ± 0.53 | 0 | 16.12 ± 0.19 |
| Mix 7 | 1 | 99 | N/A | N/A | N/A | N/A | 0 | 0 | 16.4 ± 0.15 |
| Mix 8 | 0.1 | 99.9 | N/A | N/A | N/A | N/A | 0 | 0 | 16.03 ± 0.18 |
| Mix 1 | 99.9 | N/A | 0.1 | N/A | N/A | N/A | 15.46 ± 0.19 | 0 | 17.67 ± 0.09 |
| Mix 2 | 99 | N/A | 1 | N/A | N/A | N/A | 15.68 ± 0.16 | 0 | 18 ± 0.05 |
| Mix 3 | 90 | N/A | 10 | N/A | N/A | N/A | 16.11 ± 0.34 | 19.69 ± 0.5 | 18.08 ± 0.08 |
| Mix 4 | 70 | N/A | 30 | N/A | N/A | N/A | 15.93 ± 0.27 | 16.86 ± 0.36 | 17.48 ± 0.17 |
| Mix 5 | 30 | N/A | 70 | N/A | N/A | N/A | 17.77 ± 1.3 | 16.05 ± 0.32 | 18.2 ± 0.08 |
| Mix 6 | 10 | N/A | 90 | N/A | N/A | N/A | 20.34 ± 0.51 | 15.50 ± 0.2 | 18.27 ± 0.14 |
| Mix 7 | 1 | N/A | 99 | N/A | N/A | N/A | 0 | 15.12 ± 0.19 | 17.98 ± 0.13 |
| Mix 8 | 0.1 | N/A | 99.9 | N/A | N/A | N/A | 0 | 15.02 ± 0.21 | 17.97 ± 0.08 |
| Mix 1 | N/A | N/A | N/A | N/A | 99 | 1 | 0 | 21.18 ± 0.32 | 19.78 ± 0.25 |
| Mix 2 | N/A | N/A | N/A | N/A | 95 | 5 | 0 | 21.17 ± 0.4 | 19.94 ± 0.22 |
| Mix 3 | N/A | N/A | N/A | N/A | 90 | 10 | 0 | 21.03 ± 0.48 | 19.19 ± 0.29 |
| Mix 4 | N/A | N/A | N/A | N/A | 70 | 30 | 0 | 22.28 ± 0.45 | 19.83 ± 0.29 |
| Mix 5 | N/A | N/A | N/A | N/A | 30 | 70 | 0 | 23.5 ± 0.44 | 18.39 ± 0.27 |
| Mix 6 | N/A | N/A | N/A | N/A | 10 | 90 | 0 | 23.81 ± 0.21 | 19.11 ± 0.26 |
| Mix 7 | N/A | N/A | N/A | N/A | 5 | 95 | 0 | 0 | 18.32 ± 0.23 |
| Mix 8 | N/A | N/A | N/A | N/A | 1 | 99 | 0 | 0 | 18.56 ± 0.22 |
| Mix 1 | 0.1 | N/A | N/A | 99.9 | N/A | N/A | 15.99 ± 0.82 | 0 | 24.30 ± 0.64 |
| Mix 2 | 1 | N/A | N/A | 99 | N/A | N/A | 14.15 ± 0.19 | 0 | 19.91 ± 0.36 |
| Mix 3 | 10 | N/A | N/A | 90 | N/A | N/A | 13.80 ± 0.02 | 0 | 16.04 ± 0.44 |
| Mix 4 | 30 | N/A | N/A | 70 | N/A | N/A | 13.78 ± 0.02 | 0 | 15.46 ± 0.83 |
| Mix 5 | 70 | N/A | N/A | 30 | N/A | N/A | 13.63 ± 0.02 | 0 | 14.00 ± 0.13 |
| Mix 6 | 90 | N/A | N/A | 10 | N/A | N/A | 13.57 ± 0.01 | 0 | 13.80 ± 0.09 |
| Mix 7 | 99 | N/A | N/A | 1 | N/A | N/A | 13.58 ± 0.03 | 0 | 13.80 ± 0.08 |
| Mix 8 | 99.9 | N/A | N/A | 0.1 | N/A | N/A | 13.57 ± 0.01 | 0 | 13.80 ± 0.04 |
Abbreviation: N/A, not applicable.
a
Data (average ± SD) represent 20 replicates.