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. Author manuscript; available in PMC: 2020 Dec 8.
Published in final edited form as: J Med Chem. 2019 Nov 18;62(22):10182–10203. doi: 10.1021/acs.jmedchem.9b01065

Figure 6.

Figure 6.

(a) dose responses for the reversion of EMT biomarker reporter activity in SW620 tumor organoids. (b) Representative dose response images for both vimentin and E-cadherin reporter activity. At higher doses we observed potential early stages of cell death indicated by loss of RFP reporter activity. (c) representative brightfield images indicating TOP2A inhibitors prevent SW620 tumor organoid growth compared to DMSO (scale bars = 250 μm). (d) SW620 tumor organoid volumes were measured at 72 h quantifying compound effects on tumor growth. (e) At the highest doses, all compounds display an induction of apoptosis based on cleaved caspase 3 and 7 activity with 42 and 60 at ~9-fold and ~3-, respectively.