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. 2020 Nov 30;4(1):e202000765. doi: 10.26508/lsa.202000765

Figure 1. Murine scratch wounding model follows physiological phases of wound healing.

Figure 1.

(A) Mouse model, R26-CreERT2:R26-mTmG, before Cre recombination cells ubiquitously express a membrane-localized tdTomato. Upon Tamoxifen injection, Cre is activated and randomly and dose-dependently recombines the reporter cassette from tdTomato to GFP. Therefore, some cells start to express membrane-localized GFP. (B) Schematic murine epidermis upon scratch wounding. (C) Immunofluorescence stainings immediately (0), 8, 16, and 24 h after wounding. Fibronectin (FN, magenta) indicates dermal remodeling in scratch wounds (left panel, scale bar 100 μm) or full-thickness punch biopsies (right panel, scale bar 100 μm) of E-cad-CFP mice. White bar indicates the wound site. DAPI, blue. (D) Immunofluorescence stainings detecting keratin 14 (basal cell marker, magenta), and keratin 10 (suprabasal cell marker, yellow) (top panel), with arrow pointing to a fibrin clot. Keratin 6 (stress response marker, magenta) and CD45 (leukocyte marker, yellow) (bottom panel) in the skin of E-cad-CFP mice (cyan) (representative images from n = 3 mice). Scale bar, 100 μm.