Fig 1. Selection of HD5-resistant HAdV-5.

HAdV-5 “mutator” virus was pre-bound to 293β5 cells and then incubated with HD5 to select for resistant viruses. (A) The right y-axis indicates the concentration of HD5 used for selection (solid black line). The left y-axis indicates the percentage of reads containing the denoted mutations in hexon (blue) and L4-100K (purple) in pools of virus expanded from the bulk selected population. (B) All mutations in other proteins that were found in at least 50% of the population during selection. (C) HD5 IC₅₀s of pools of virus expanded from the bulk selected population were determined on 293β5 cells. Each point is an independent experiment, and linear regression with 95% confidence bands is graphed.