Skip to main content
. 2020 Dec 8;18(12):e3000703. doi: 10.1371/journal.pbio.3000703

Fig 4. Rescue of the endosome enlargement.

Fig 4

(a–a”‘) TEM horizontal sections of the cortical region of 7-day-old fly brains showing neuronal cell bodies (circled in blue) and the early endosome–like vesicles marked with a red arrow. Each panel is a different genotype: (a) control,;; nsybGal4, (a’) Appl-/-, Appld;;nsyb Gal4, (a”) reexpression of the flAPPL, Appld; UAS flAPPL; nsybGal4, and (a”‘) only the SAPPL Appld; UAS SAPPL; nsybGal4 individually in an APPL null background. n = 3 to 5 brains per genotype and approximately 60 cells analyzed per genotype. (b) This graph represents the quantification of the size of the early-endosome–like vesicles. Reexpressing the flAPPL rescued the increased size of early-endosome–like vesicles seen in Appld;;nsybgal4 flies, n = 3 to 5 brains per genotype and approximately 60 cells analyzed per genotype *p = 0.0191, ***p = 0.0005. (c) The significantly higher percentage of cells containing early-endosome–like vesicles was also rescued when reexpressing the flAPPL and only the SAPPL, Fisher exact test ****p < 0.0001. Underlying data can be found in the S1 Data file. APPL, APP Like; flAPPL, full-length APPL; ns, not significant; SAPPL, secreted portion of APPL; TEM, transmission electron microscopy.