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. 2020 Nov 17;47(1):335–345. doi: 10.3892/ijmm.2020.4789

Figure 2.

Figure 2

Resveratrol promotes G1/S phase arrest of cervical cancer cells. (A) HeLa cells and (B) Ca Ski cells were treated with resveratrol at various concentrations (0, 10, 20 and 40 µM) and the vehicle (DMEM medium supplemented with 10% fetal bovine serum, and containing 1/2,500 volume of ethanol) for 24 h. Cell cycle was determined by flow cytometry. Cell cycle curve fitting was completed by using the cell cycle function of FlowJo™ v10.7 (Version X). (C) p16, p21 and p27 mRNA levels in HeLa cells treated with resveratrol or vehicle for 24 h were determined by RT-qPCR. (D) p16, p21, p27, CDK4, Cyclin E, E2F1 and p-pRb1 protein levels in HeLa cells treated with resveratrol or the vehicle for 24 h were determined by western blot analysis and quantitative analysis of western blot analysis results. Data are presented as the means ± SD. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, compared with the vehicle control.