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. 2020 Nov 17;47(1):335–345. doi: 10.3892/ijmm.2020.4789

Figure 3.

Figure 3

Resveratrol inhibits the expression of HPV18-E6 and HPV18-E7 in cervical cancer cells. (A) HPV18 E6/E7, p53 and p-pRb1 levels in HeLa cells treated with resveratrol (40 µM) or the vehicle (DMEM medium supplemented with 10% fetal bovine serum, and containing 1/2,500 volume of ethanol) for 24 h were determined using immunocytochemistry. (B) Quantitative results of (A). (C) HPV18 E6/E7 protein levels in HeLa cells treated with resveratrol (40 µM) or the vehicle for 24 h were determined by western blot analysis. (D) HPV18 E6/E7 mRNA levels in Ca Ski cells treated with resveratrol (50 µM) or the vehiclefor 24 h were determined by RT-qPCR. (E) HPV18-E6/E7, p53 and p-pRb1 protein levels in Ca Ski cells treated with resveratrol (50 µM) or the vehicle for 24 h were determined by western blot analysis. Data are presented as the means ± SD. *P<0.05, **P<0.01, ***P<0.001, compared with the vehicle control.