FIGURE 3.

Danhong injection (DHI) improves reparative potency of DHI for myocardium in rats after ischemia/reperfusion (I/R). (A) Sections collected from the border zones of infarction were stained for TUNEL (green, bar = 100 μm) to identify apoptotic cells, Ki67 (red, bar = 100 μm) to identify cell proliferation and DHE (red, bar = 75 μm) to identify reactive oxygen species generation. (B) Apoptosis was quantified as the percentage of TUNEL-positive cells. Cardiomyocytes were visualized via staining for troponin I (red), and nuclei were counterstained with DAPI (blue). (C) Cardiomyocyte proliferation was quantified as the percentage of Ki67 and Troponin I-positive cells (green), and nuclei were counterstained with DAPI (blue). (D) DHE fluorescence intensity was quantified. n (number of animals) = 4,*p < 0.05 or**p < 0.01 vs. I/R group.