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. 2020 Nov 11;47(1):171–182. doi: 10.3892/ijmm.2020.4788

Figure 3.

Figure 3

Efficiency of transfection of hBMSCs with LINC00473 and PEBP1. (A-a) hBMSCs expressing GFP tagged LINC00473-up lentivirus under a fluorescence microscope (scale bar, 1,000 µm) and (A-b) under a light microscope (scale bar, 1,000 µm). The results of RT-qPCR revealed that (A-c) the relative expression of LINC00473 was significantly upregulated by LINC00473-up lentivirus (494%), *P<0.05 compared with the normal group and vector group, and (B-a) the relative expression of PEBP1 was significantly downregulated by 3 shRNAs for PEBP1 (sh-PEBP1), *P<0.05 compared with the normal group and sh-Control group, and (B-b) the relative expression of PEBP1 was a significantly upregulated by PEBP1 overexpression vector (PEBP1-up), *P<0.05 compared with the normal group and PEBP1-Control group. The results of western blot analysis revealed that (C-a) the relative expression of PEBP1 protein was significantly downregulated by the 3 sh-PEBP1, *P<0.05 compared with the normal group and sh-Control group, and (C-b) the relative expression of PEBP1 protein was a significantly upregulated by PEBP1-up, *P<0.05 compared with the normal group and PEBP1-Control group. All data were presented as the means ± standard deviation. hBMSCs, human bone marrow-derived mesenchymal stem cells; LINC00473, lncCRNA-00473; PEBP1, phosphatidylethanol-amine-binding protein 1; GFP, green fluorescent protein; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.