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. 2020 Nov 11;47(1):171–182. doi: 10.3892/ijmm.2020.4788

Figure 7.

Figure 7

PEBP1 mediates the activation of the Akt/Bad/Bcl-2 signaling pathway by LINC00473 in Dex-stimulated hBMSCs. The result of western blot analysis revealed (Aa and b) the protein expression levels of PEBP1, p-Akt and Akt in each groups following infection with LINC00473-up lentivirus in Dex-induced hBMSCs (*P<0.05 compared with the Dex group and Dex + Vector group), and (Ba and b) the protein expression levels of PEBP1 and p-Akt in each groups following co-transfection with LINC00473 and PEBP1 in Dex-stimulated hBMSCs (*P<0.05 compared with the Dex + LINC00473-up group and Dex + LINC00473-up + sh-Control group), and (Ca and b) the protein expression levels of PEBP1 and p-Akt in each group following transfection with LINC00473 and PEBP1 respectively in Dex-induced hBMSCs (*P<0.05 compared with the Dex group, Dex + Vector group and Dex + PEBP1-Control group). (D) The results of RT-qPCR revaled the PEBP1 mRNA expression in each groups (*P<0.05 compared with the Dex group and Dex + Vector group, #P<0.05 compared with the Dex + LINC00473-up group, &P<0.05 compared with the Dex group and Dex + PEBP1-Control group). All data are presented as the mean values ± standard deviation 3 three independent experiments. PEBP1, phosphatidylethanolamine-binding protein 1; Akt, protein kinase B; Bad, Bcl-2-associated death promoter; Bcl-2, B-cell lymphoma 2; LINC00473, lncRNA-00473; hBMSCs, human bone marrow-derived mesenchymal stem cells; Dex, dexamethasone; p-Akt, phosphorylated Akt; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.