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. 2020 Nov;8(21):1400. doi: 10.21037/atm-20-1598

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2020 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Formation of endothelialized hepatic cell aggregates in a polydimethylsiloxane (PDMS)-based oxygen-permeable microwell device. (A) Structure of the PDMS-based oxygen-permeable microwell device. (B) Detailed structure of a microwell membrane. (C) Schematic showing the process of co-culturing hepatocellular carcinoma (HepG2) cells, Swiss 3T3 cells, and human umbilical vein endothelial cells (HUVECs) in the microwell device.