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. 2020 Nov;8(21):1364. doi: 10.21037/atm-20-3661

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2020 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Morphological characteristics of the AEM fabricated with conventional or optimized protocol. (A) Gross features, cellular components, collagens distribution and content, GAGs distribution and content of the NET, C-AEM or O-AEM. H&E and DAPI for observing cellular components. PR together SR-FTIR for evaluating the distribution and content of collagen. TB combined with SR-FTIR for evaluating the distribution and content of GAGs. Bar =100 µm; (B) collagen and GAGs contents in the BR, FR or TR of the NET, C-AEM or O-AEM (n=6); (C) DNA content in the NET and the two kinds of AEM (n=5). *, P<0.05; **, P<0.01; ***, P<0.001. AEM, acellular enthesis matrix; GAG, glycosaminoglycan; NET, native enthesis tissue; H&E, hematoxylin and eosi; DAPI, 4',6-diamidino-2-phenylindole; PR, picrosirius red; SR-FTIR, synchrotron-radiation Fourier-transform infrared spectroscopy; TB, toluidine blue; BR, bony region; FR, fibrocartilaginous region; TR, tendinous region.