Figure 4.

M2b macrophages inhibited the kinase activation of PDGFRs of CFs. CFs were co-cultured with M2b macrophages or cultured with M2b macrophage supernatant or the same volume of culture medium (blank control, NT). (A) Expression of α-SMA evaluated with immunofluorescence staining (400×); (B) expression of α-SMA and CCN2 with western blot analysis; (C) expression and phosphorylation of PDGFRs in CFs with western blot analysis. The data in (B) and (C) are representative of three independent experiments. *, P˂0.05; **, P˂0.01. PDGFR, platelet-derived growth factor receptor; CF, cardiac fibroblast.