Table 1.
Primer sequences for related genes.
| Gene | Primer Sequences (5′–3′) |
|---|---|
| Alkaline phosphatase (ALP) | Forward primer: TGCAGGATCGGAACGTCAAT |
| Reverse primer: GAGTTGGTAAGGCAGGGTCC | |
| Osteocalcin (OCN) | Forward primer: TCCCTCTCTCTGCCTCGAAA |
| Reverse primer: GGGCTGTCTGGGATTGAACA | |
| Osteopontin (OPN) | Forward primer: GGGATCGCTGAAGGCATCAA |
| Reverse primer: CAGCCGTCAAAGGCTTCAAA | |
| RUNX2 | Forward primer: CACAAGTGCGGTGCAAACTT |
| Reverse primer: AATGACTCGGTTGGTCTCGG | |
| Notch1 | Forward primer: TTGGTCCGAGGGCATCTCTA |
| Reverse primer: ACAGAGCTTGGGAACGGAAG | |
| Jagged1 | Forward primer: CTGCTTGAATGGGGGTCACT |
| Reverse primer: CACGATTGTAGCATTGGGCG | |
| Hes1 | Forward primer: CAACACGACACCGGACAAAC |
| Reverse primer: CGGAGGTGCTTCACTGTCAT | |
| Hes5 | Forward primer: GTGGAGATGCTCAGTCCCAA |
| Reverse primer: TCAATGCTGCTGTTGATGCG | |
| GAPDH | Forward primer: AGTGCCAGCCTCGTCTCATA |
| Reverse primer: GGGTTTCCCGTTGATGACCA |