Figure 1.

The synthetic RNA oligonucleotide ICR2 improves the effectiveness of fibroblast reprogramming to cardiomyocytes

Fibroblasts were transfected with either the reprogramming cocktail miR combo or a non-targeting control miR (negmiR). 1 day later, transfection complexes were removed and the cells cultured for 4 days with the ED100 dose of ICR2 or ICR4 (5 μg/mL). 14 days after transfection, RNA was analyzed for the expression of the indicated cardiomyocyte-specific genes. Expression values were normalized to the housekeeping gene GAPDH and are represented as a fold change compared to the non-targeting control miR negmiR. N = 15. ∗Comparisons made to negmiR, ∗∗∗p < 0.001. †Comparisons made between miR combo + ICR and miR combo + vehicle, †† p < 0.01.