Figure 1.

Construction of Gene-Silencing Oncolytic Adenoviruses

(A) Schematic strategy for rapid production of oncolytic adenoviruses with incorporated RNAi molecule expression cassette. (B) RNA transcript sequences of the three different miR-1 precursor designs. Mature and passenger strand sequences are shown in red and blue, respectively. (C and D) Predicted optimal secondary folding structures of adenovirus-encoded hsa-pri-miR-1 (C) and hsa-pri-miR-26b (D), with the microRNA stem-loops pointing down. Predicted structures are based on minimum free energy and base-pairing probability calculation using RNAfold WebServer.¹⁶ The positions of the mature miRNA sequences are indicated.