Skip to main content
. 2020 Oct 27;19:332–343. doi: 10.1016/j.omto.2020.10.012

Figure 2.

Figure 2

Analysis of Oncolytic Adenoviruses Expressing Different MicroRNA Precursor Molecules

(A) Dose-response cytotoxicity analysis of oncolytic adenoviruses expressing different miR-1 precursor molecules on A549 lung cancer cells. Data shown are means of two independent experiments in triplicate. (B) Quantitative RT-PCR analysis of mature miR-1-3p expression in HCT116 parental and Drosha knockout (KO) colorectal carcinoma cells infected with oncolytic adenoviruses expressing different miR-1 precursor molecules. (C) Expression of mature miR-1-3p in different cancer cell lines infected with AdΔ24E3-U6 or AdΔ24E3-U6.pri-miR-1. (D) Time course analysis of mature miR-1-3p expression in A549 cells infected with oncolytic adenoviruses expressing different miR-1 precursor molecules. (E) Comparison of mature miR-1-3p expression in HCT116 cells infected with AdΔ24E3-U6 or AdΔ24E3-U6.pri-miR-1, before and after 6 weeks of virus propagation. Data shown in (B)–(E) are means of three independent experiments with cells infected at 100 IU/cell in duplicate and are given relative to uninfected cells.