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. 2020 Nov 25;11:579389. doi: 10.3389/fmicb.2020.579389

TABLE 1.

Oligonucleotides used for PCR.

Gene Primer Sequence (5′–3′) Annealing temperature (°C) PCR product (bp) References
FliL FliL1 CTCTGCTCGTGGTGGTGTCG 57 770 Barbour et al., 2012
FliL2 GCGTCGTCACCTGATGTGTC
mrpA mrpA1 ACACCTGCCCATATGGAAGATACTGGTACA 40 550 Barbour et al., 2012
mrpA2 AAGTGATGAAGCTTAGTGATGGTGATGGTGATGAGAGTAAGTCACC
pmfA pmfA1 TCAGCATGTGGATTAGCAGCA 59 385 This study
pmfA2 CTTGGAATTCACCTGGCGTT
hpmA hpmA1 GTTGAGGGGCGTTATCAAGAGTC 55 709 Cestari et al., 2013
hpmA2 GATAACTGTTTTGCCCTTTTGTGC
hpmB hpmB1 CAGTGGATTAAGCGCAAATG 55 422 Cestari et al., 2013
hpmB2 CCTTCAATACGTTCAACAAACC
Uca uca1 GCTGGCTCATCTATGGCGTA 60 453 This study
uca2 AGCGGTAGATTGTCCGGTTG
ureC ureC1 TGGCAAGGCAGGTAATCCAG 58 589 This study
ureC2 ATTGGGCTCTCCTACCGACT
zapA zapA1 TGGCGCAAATACGACTACCA 57 332 This study
zapA2 TATCGTCTCCTTCGCCTCCA