. 2020 Dec 1;75:71–143. doi: 10.3897/mycokeys.75.59872

Table 3.

Summary of polymorphic regions in ITS1 and ITS2 spacers assessed in Ganoderma species/groups represented by ≥ 10 entries in the GenBank/ENA/DDBJ and UNITE databases. For each region, the length and position between conserved oligonucleotides (i.e. TGCAC to GAATG in ITS1 and AATCT to TAGCT in ITS2) are hereby provided. Additional pertinent information is included in Suppl. material 2: Fig. S1. Grouping in Clades/Clusters, as well as names and number of sequences per name, are in accordance with Table 1 and Fig. 3.

Species/Groups	ITS1 sequence of potential diagnostic value	Length (nt)	Position in the alignment of Suppl. material 2: Fig. S1 (without gaps)	ITS2 sequence of potential diagnostic value	Length (nt)	Position in the alignment of Suppl. material 2: Fig. S1 (without gaps)
CLADE A
Cluster A.1
G. oregonense		13	25–37	GCCTTTGCGGGTW	26	17–42
G. tsugae	TGTGAAGCGTGCT	13	25/26–37/38	TGYRGGCTTGGAC	26	17–42
G. carnosum		13	25–37	AGCCTTGC	8	16–23
G. lucidum	TGAAGCGYNCCYY	13	27–39	nd
G. leucocontextum – G. weixiensis	CGAAGCGTGC	10	27–36	nd
Cluster A.2
G. hoehnelianum	CTTCAGTC	8	16–23	CTTGTGGGTT	10	20–29
G. weberianum	nd			nd
G. sichuanense	nd			nd
G. carocalcareum	AACGTCGTKAAGCGGGC	17	21–37	nd
Ganoderma sp. A1	GGGTCTTTT	9	34–42	CGTCTTTC	8	60–67
G. mexicanum	GCTCTTTACTGAGCC	15	36–50	CGGCCGGCTCCTCT	21	65/67–85/87
G. parvulum		15	36–50	TAAATGC¹	21	65/67–85/87
G. resinaceum	AAGCGGCG	8	55/56–62/63	nd
Ganoderma sp. A3	GGATCGGCGT	10	55–64	ACAGATCT	8	13–20
G. polychromum	ACACCTAT	8	84–91	nd
G. sessile	CCACAAACTCTR	12	78–89	CTTACAAA	8	10–17
Cluster A.3
G. tuberculosum	GATTGTCG	8	21–28	CCATGCCC	8	58/59–65/66
G. wiiroense	GGCATTAT	8	21–28	TTCTCTTA	8	71/72–78/79
G. philippii	TTGCTGGG	8	39–46	CTTTTGTGGYTTT	13	18–30
G. lingzhi	CAGATTGC	8	19–26		10	54–63
G. curtisii	TGCGGAGCGCAT	12	49–60	CGGCCGTTAT	10	54–63
G. ravenelii	GAGTGCAT	8	53–60		10	54–63
G. multiplicatum	CCCTTTAT	8	35–42	nd
G. destructans – G. dunense		9	22–30	nd
G. steyaertanum	ATCVTAAAA²	9	22–30	CTCTTGGCC	9	61–69
G. martinicense		9	22–30	CATTCTTG	8	59–66
G. multipileum		9	22–30	G(C)AAGCTTTTG	10–11	13–22/23
Ganoderma sp. A6	TCCCAGGA	8	50–56	CTCCTCTCTT	10	72–81
G. tropicum	ACCGGGCTTTGCA	13	42–54	nd
CLADE B
G. applanatum	GTGCTYTT	8	32–39	TAAGCTTKTGT	11	14–24
CLADE C
G. neojaponicum	ATGGATCGCG	10	18–27	AGGTGTTTG	9	47–55
G. enigmaticum – G. thailandicum	CTTCTTGTC	9	35–43	TTGCAACC	8	11–18
G. casuarinicola	GCTCTTGT	8	34–41		8	11–18
CLADE D
G. mbrekobenum	TTWCAGASSGT	11	16–26	AGGCTATT	8	48–55
G. nasalanense	CGTTTTCA	8	70–77	TCTTTAATA	9	60/62–68/70
G. sinense	GGAGCTSGT	9	41–49	GTAAAGGC	8	24–31
G. mastoporum	nd			TTTTTARYGRKTTTGTAGG	19	19–37
G. angustisporum	GTGTAAAA	8	27–34	ATGGCTWGT	8	24/28/29–32/36/37
G. zonatum	TCGCTCGC	8	34–41	TCTCTTCA	8	3–10
G. ryvardenii	TCGTGCGG	8	23–30	CTTTAACT	8	61–68
G. boninense	GTTTGACRAGTT	12	40/44–51/55	ATCTCTTTGY	10	16–25
Ganoderma sp. D3	GGCGTGGT	8	24–31		10	16–25
CLADE E
G. williamsianum	CTTCAGGTC	9	16–24	CTTAATYGA	9	21–29
Ganoderma sp. E1	GTTTTACG	8	15–22	ATRAGCTTCT	10	13–22
Ganoderma sp. E2		8	15–22	TATGKGAG	8	23–30
G. aff. gibbosum		13	27–39		10	60–69
G. gibbosum	TGARRSGGGCTYG³	13	27–39	TCCYTTTACR³	10	60–69
G. ellipsoideum		13	27–39		10	60–69
Ganoderma sp. E4	RTTAAACG	8	26–33	GTCGGACTW⁴	9	59–67
G. pfeifferi	GGCCCGTTT⁵	9	34/35–42/43	GCCTTTGTC⁶	9	57–65
Ganoderma sp. E6	ACYGAGCYYGC	11	41–51	TCTTTGCGGGG	11	19–29
G. australe	CGAAACGKGCTCG	13	27–39		11	19–29
Ganoderma sp. E7	CCCCATGA	8	83/84–90/91	GTCTTTACA	9	59–67
G. aff. adspersum	GGGCCCGTTC	10	33–42	CTTCTTGCGG	10	18–27
G. adspersum	AGGCCCGTTC	10	33–42	AGGTTTGTAGGG	12	27/28–38/39