Figure 1.

General methodology for the initial screen of the ReFRAME library and Dose-response assays using the 96-well microtiter plate model of Candida biofilm formation. (A) The initial screen was performed at a final concentration of 5 µM of each compound in the library. The plate map of the 96 well plates is as follows: 1 denotes wells with no compound to serve as growth controls for each plate, 2 denotes wells with test compounds, and 3 denotes wells with Amphotericin B at 4 µg/ml to serve as the positive control for inhibition. (B) The dose-response plates were used to confirm hits from the initial screen and establish their potency. Hits from the initial screen were tested in a series of concentrations ranging from 20 to 0.00103 µM. For each hit compound, serial dilutions are represented by the changing colors numbered 3 to 10, as seen for example for one hit compound from well A1 to A8. Wells labeled 2 in column 11 contained Amphotericin B at a final concentration of 4 µg/ml, and wells labeled 1 in column 12 contained no compound to serve as growth controls.