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. 2020 Nov 16;56(10):866–877. doi: 10.1007/s11626-020-00518-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Figure 1. — Effects of miRNA-23a-3p overexpression on cell proliferation in HG-treated H9c2 cells. (a) RT-qPCR to detect the expression level of miRNA-23a-3p in HG-treated H9c2 cells (****p < 0.0001 vs. NG group). (b) H9c2 cells were exposed to glucose at concentrations of 5 mM (NG), 35 mM (HG), and 5 mM glucose + 30 mM mannitol (NG + Mannitol) for 24, 48, and 72 h (****p < 0.0001 vs. NG group). Cell viability was assessed by a CCK-8 assay. (c) H9c2 cell viability was significantly increased following treatment with the miRNA-23a-3p mimics (**p < 0.01 vs. NC-mimics). (d) Representative images illustrating the EdU and Hoechst staining of H9c2 cells exposed to 5 mM and 35 mM glucose and transfected with NC mimics or miRNA-23a-3p mimics. (e) Analysis of EdU-positive cardiomyocytes by an EdU incorporation assay. The percentages of proliferative H9c2 cells were calculated (n > 500). Compared with the NC-mimics group, the miRNA-23a-3p-mimics group had increased EdU incorporation (**p < 0.01). CCK-8 Cell Counting Kit-8, EdU 5-ethynyl-2′-deoxyuridine, NG normal glucose, HG high glucose, NC negative control.