Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Sequence-specific binding of TyrR to the cpxR-cpxP promoter region. Electrophoretic mobility shift assays (EMSAs) were performed with 100 ng of 6-FAM-labeled DNA fragments spanning the wild-type cpxR-cpxP intergenic region or the same region containing point mutations in predicted TyrR binding sites that overlap with CpxR box 1, 2, or 3 (Fig. 7) and with increasing concentrations (0 to 4.39 μM) of purified TyrR. F, location of free unbound DNA.
