FIG 3.
Activation of dmpM expression requires aromatic amino acids and TyrR. Relative expression of dmpM was determined in the E. ludwigii wild-type strain (A) and the isogenic tyrR mutant (B) grown in M9 minimal medium supplemented with or without 1 mM each aromatic amino acid by quantitative RT-PCR. Relative expression was determined using the ΔΔCT method by comparing to the respective wild-type transcripts in M9 minimal medium alone (A) or to the wild-type transcripts under each growth condition (B). Expression was normalized to recA expression levels. Data represent the average from at least three biological replicates; error bars represent the standard error of the mean. The asterisks indicate significant differences (P < 0.05) from the control treatment.