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Sequence-specific binding of TyrR to the dmpM promoter. Electrophoretic mobility shift assays (EMSAs) were performed with 100 ng of 6-FAM-labeled DNA fragments spanning the wild-type dmpM promoter region or the same region containing point mutations in predicted TyrR boxes 1 and/or 2 (Fig. 4), and increasing concentrations (0.44, 0.87, 2.19, and 4.39 μM) of purified TyrR. The location of free unbound DNA (F) is indicated.
