TABLE 2.
Primers used in this study
| Primer | Sequence (5′–3′) |
|---|---|
| GalR F | AAGACAAGCCAGAACCATTTGGG |
| GalR Ery F | CGGGAGGAAATAATTCTATGAGGAAAGTACCCTAAATCAAGAATAG |
| GalR R | GACAAGGTTGTTGTTATCGGTGAT |
| GalR Ery R | TTGTTCATGTAATCACTCCTTCTGTGCAATGTCTTTTAAGGTAGCC |
| LacD F | CTGTATCGCTATTCTCCACG |
| LacD Ery F | CGGGAGGAAATAATTCTATGAGGGTATCATCTCAGCTCTTGC |
| LacD R | TGATCTGCTAGCTTCTGAC |
| LacD Ery R | TTGTTCATGTAATCACTCCTTCGCAAGAGCTGAGATGATACC |
| Ery F | GAAGGAGTGATTACATGAACAA |
| Ery R | CTCATAGAATTATTTCCTCCCG |
| GalK F | CCTTCATTAAGTCATAGCCAGA |
| GalK Spec F | AAATAACAGATTGAAGAAGGTATAAGAAGTAGTTGGATACGCTCC |
| GalK R | GTAGGACAGACATTGGCCA |
| GalK Spec R | TATGTATTCATATATATCCTCCTCCAAATTGATACGACCTGGTG |
| LacAB F | AATATCGGACAAGCTGGT |
| LacAB Spec F | AAATAACAGATTGAAGAAGGTATAAGCTCAACAAACAGACGC |
| LacAB R | TTCGTCTGAGCTATCTACATC |
| LacAB Spec R | TATGTATTCATATATATCCTCCTCATCTCAAACCTGCAGCATC |
| LacD F | CTGTATCGCTATTCTCCACG |
| LacD Spec F | AAATAACAGATTGAAGAAGGTATAATGAAGCAGCAGCTCGCGAAT |
| LacD R | TATGTATTCATATATATCCTCCTCGCAAGAGCTGAGATGATACC |
| LacD Spec R | GTTGCGTAATCACATCATAGAT |
| J253 | GAGGAGGATATATATGAATACATACG |
| J254 | TTATACCTTCTTCAATCTGTTATTTAAATAGTTTATAGTTA |
| AAA F | ACTCCACGGTCGCAAAATTCCTGCGCTGGCCATGCTGGGAGCCAGACTGACATTAAGA |
| AAA R | TCTTAATGTCAGTCTGGCTCCCAGCATGGCCAGCGCAGGAATTTTGCGACCGTGGAGT |
| ATT F | AGTACTCCACGGTCGCAAAATTCCTGCCCTGACCATGCTGGGAACCAGACTGACATTAAGAGAAAGTACCC |
| ATT R | GGGTACTTTCTCTTAATGTCAGTCTGGTTCCCAGCATGGTCAGGGCAGGAATTTTGCGACCGTGGAGTACT |
| SAT F | AGTACTCCACGGTCGCAAAATTCCTAGCCTGGCCATGCTGGGAACCAGACTGACATTAAGAGAAAGTACCC |
| SAT R | GGGTACTTTCTCTTAATGTCAGTCTGGTTCCCAGCATGGCCAGGCTAGGAATTTTGCGACCGTGGAGTACT |
| STA F | AGTACTCCACGGTCGCAAAATTCCTAGCCTGACCATGCTGGGAGCCAGACTGACATTAAGAGAAAGTACCC |
| STA R | GGGTACTTTCTCTTAATGTCAGTCTGGCTCCCAGCATGGTCAGGCTAGGAATTTTGCGACCGTGGAGTACT |
| AAT F | AGTACTCCACGGTCGCAAAATTCCTGCCCTGGCCATGCTGGGAACCAGACTGACATTAAGAGAAAGTACCC |
| AAT R | GGGTACTTTCTCTTAATGTCAGTCTGGTTCCCAGCATGGCCAGGGCAGGAATTTTGCGACCGTGGAGTACT |
| ATA F | AGTACTCCACGGTCGCAAAATTCCTGCCCTGACCATGCTGGGAGCCAGACTGACATTAAGAGAAAGTACCC |
| ATA R | GGGTACTTTCTCTTAATGTCAGTCTGGCTCCCAGCATGGTCAGGGCAGGAATTTTGCGACCGTGGAGTACT |
| SAA R | AGTACTCCACGGTCGCAAAATTCCTAGCCTGGCCATGCTGGGAGCCAGACTGACATTAAGAGAAAGTACCC |
| SAA R | GGGTACTTTCTCTTAATGTCAGTCTGGCTCCCAGCATGGCCAGGCTAGGAATTTTGCGACCGTGGAGTACT |
| gyr-RT-F | ACTGGTATCGCGGTTGGGAT |
| gyr-RT-R | ACCTGATTTCCCCATGCAA |
| galR-RT-F | TCTCTATCGCCGACCGTATCC |
| galR-RT-R | GGTGTAGCCCAGCTCTTCAG |
| galK-RT-F | CACGTTTCTCTGGAGCATGA |
| galK-RT-R | ATGGCACAGCCACTAAAACC |
| galT-RT-F | GTGGGAGAAGGTGTTTTGGA |
| galT-RT-R | ACGCGCAGTCTGACTATCCT |
| lacA-RT-F | CGTGATTGATGCTTATGGAG |
| lacA-RT-R | AGCCAATTCATCACCAACAAG |
| lacD-RT-F | CATCGGTTCTGAGTGTGTGG |
| lacD-RT-R | AAAGCGTGGGTCTGAAAAGA |
| galR–Seq F | AATCTATCATGATGAACTGGTC |
| galR–Seq R | CATAATGGAGGGCGTATGG |