FIG 4.
Microscale thermophoresis measurements of a preincubated SRP-FtsY complex with RNCs. Unlabeled SRP-FtsY (500/250 nM to 0.24/0.12 nM) was mixed with 5 nM labeled RNCs, incubated for 5 min on ice, and filled into premium capillaries (NanoTemper Technologies). (A) The change in normalized fluorescence (ΔFnorm) of FtsQ4-85 and Luc2-50 is plotted against the concentration of unlabeled SRP-FtsY. (B) The change in normalized fluorescence (ΔFnorm) of SciP2-54, SciP54-100, and SciP54-100 C68S is plotted against the concentration of unlabeled SRP-FtsY. (C) Summary of the Kd values (three independently pipetted measurements are merged, and the error bars represent the standard deviation). The raw data of the MST measurements are shown in Fig. S3, Fig. S4, and Table S2.