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. 2020 Dec 8;10:426. doi: 10.1038/s41398-020-01108-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Brain samples were collected from P20 to P24. a In the cerebral cortex, the GABA concentration in Gad1^−/− rats was reduced to 51.88% of that of Gad1^+/+ (F(2, 18) = 16.748, p < 0.001). b There were no differences in the glutamate (Glu) concentrations among the three genotypes in the cerebral cortex (F(2, 18) = 1.4734, p = 0.2555). c The GABA/Glu ratio was reduced in both Gad1^+/− and Gad1^−/− rats in the cerebral cortex (F(2, 18) = 10.578, p < 0.001). d GABA concentrations in the cerebellum (F(2, 18) = 29.646, p < 0.001). e Glu concentrations in the cerebellum (F(2, 18) = 0.0372, p = 0.9636). f GABA/Glu ratios in the cerebellum (F(2, 18) = 218.79, p < 0.001). n = 7 in each genotype. The results are presented as the average ± SEM. The data were analyzed using one-way ANOVA and post hoc test adjusted by Holm’s method for multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.001.